Recombinant Human Mononucleosomes (H3.1 ΔN32), Biotinylated
Cat.No. : | NUC-008 |
Product Overview : | Mononucleosomes assembled from recombinant human histones expressed in E. coli. |
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Description : | Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3.1 and H4. Accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805), with the amino acid sequence of H3 begining with glycine 33 (amino acids 1-32 are deleted), wrapped by 147 base pairs of 601 positioning sequence DNA. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5' biotin-TEG group. |
Source : | E. coli |
Species : | Human |
Tag : | N/A |
Form : | Mononucleosomes (H3.1 ΔN32), Recombinant Human (27.4 µg protein weight, 50 µg DNA+protein) in 10 mM Tris HCl, pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. |
Molecular Mass : | 193,951.1 Da |
Applications : | Mononucleosomes (H3.1 ΔN32), Human Recombinant Biotinylated are highly purified and suitable for use as substrates in enzyme screening assays, structural studies, or effector protein binding experiments. The N-terminal deletion allows for the study of the role of the N-terminus in many aspects of chromatin biology. |
Storage : | Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws. |
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◆ Recombinant Protein | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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