OncoStat Panel
Cat.No. : | NUC-063 |
Product Overview : | A panel of distinctly modified mononucleosomes assembled from recombinant human histones expressed in E. coli. |
- Specification
- Gene Information
- Related Products
- Download
Description : | A panel of distinctly modified mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3.3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.3-P84243*; H4-P62805) wrapped by 147 base pairs of barcoded Widom 601 positioning sequence DNA. The OncoStat panel is comprised of a pool of 1 unmodified (H3.3) plus 7 histone H3.3 point mutations: H3.3K4M, H3.3K9M, H3.3K27M, H3.3G34R, H3.3G34V, H3.3G34W, H3.3K36M. Each distinctly modified nucleosome is distinguishable by a unique sequence of DNA (“barcode”) at the 3’ end that can be deciphered by qPCR or next-generation sequencing. Each of the 8 nucleosomes in the pool is wrapped by 2 DNA species, each containing a distinct barcode (”A” and “B”, see SNAP-ChIP Manual) allowing for an internal technical replicate. |
Source : | E. coli |
Species : | Human |
Tag : | N/A |
Form : | Purified recombinant mononucleosomes, containing a mixture of 16 (1 unmodified plus 15 unique) H3 and H4 Purified recombinant mononucleosomes, containing a mixtureof 8 (1 unmodified plus 7 point mutations) nucleosomes in 10mM sodium cacodylate, pH 7.5, 100 mM NaCl, 1 mM EDTA,50% glycerol (w/v), 1x Protease Inhibitor cocktail, 100 ug/mLBSA, 10 mM β-mercaptoethanol. Molarity = 0.6 nM. |
Molecular Mass : | ~199283.7 Da (average MW of all 8 nucleosomes) |
Applications : | SNAP-ChIP OncoStats are highly purified recombinantmononucleosomes and are suitable for use as spike-in controlsfor ChIP reactions, for antibody specificity testing or for effectorprotein binding experiments. See manual for more information. |
Storage : | Stable for six months at -20°C from date of receipt. |
Products Types
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (0)
Ask a questionAsk a Question for All NUC Products
Required fields are marked with *
My Review for All NUC Products
Required fields are marked with *
0
Inquiry Basket