Recombinant Human Mononucleosomes (H3.3)

• Cat.No.: NUC-044
• Product Overview: Mononucleosomes assembled from recombinant human histones expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4. Accession numbers: H2A-P04908; H2B-O60814; H3.3-P84243; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. The nucleosome is the basic subunit of chromatin. H3.3 is a histone variant, a non-allelelic replacement histone found in regions of high chromatin turnover outside of S-phase (e.g. at actively transcribed genes). H3.3-H4 associates in vivo with the HIRA chaperone complex.
• Form: Purified recombinant mononucleosomes (100 µg total mass, protein+DNA, 54.6 µg protein by) in 10 mM Tris-HCl pH 7.5, 1 mM EDTA, 25 mM NaCl, 2 mM DTT, & 20% glycerol.
• Molecular Mass: 200,253 Da
• Applications: Mononucleosomes (H3.3), Human Recombinant are highly purified and are suitable for use as substrates in enzyme screening assays or for nucleosome binding experiments. The absence of post-translational histone modifications makes them ideal for conducting enzyme activity and screening assays. Mononucleosomes (H3.3), Human Recombinant do not contain free DNA which could alter assayed activities.
• Storage: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.