PAF Acetylhydrolase (PAF-AH) Inhibitor Screening Kit (Colorimetric)
Cat.No. : | Kit-2359 |
Product Overview : | Platelet-Activating Factor (PAF or 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) is an important phospholipid mediator, which has diverse biological activities. PAF is synthesized and secreted by a variety of cells such as mast cells, monocytes, macrophages etc. Up-regulated PAF signaling can cause pathological inflammation and also has been found to be responsible for sepsis, shock, and traumatic injury. PAF-Acetylhydrolase (PAF-AH or 1-alkyl-2-acetylglycerophosphocholine esterase or Lipoprotein-associated Phospholipase A2 or Lp-PLA2) (EC 3.1.1.47) hydrolyzes PAF by removing acetyl group at the sn-2 position and converts PAF into biologically inactive form, lyso-PAF. PAF-AH has two forms: extracellular and intracellular that shares some similarities. In humans, PAF-AH deficiency leads to severe asthma, thus development of novel and specific inhibitors of PAF-AH is critical for therapeutic purposes. In PAF-AH Inhibitor Screening Kit, PAF-AH hydrolyzes the acetyl thioester bond at sn-2 position of substrate and free thiols are detected using DTNB. In the presence of PAF-AH inhibitor, the reaction is impeded. The PAF-AH Inhibitor Control is included to compare the efficacy of test inhibitors. The assay is high-throughput adaptable and can be finished in less than 1 hr. |
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Applications : | Screening/studying/characterizing potential PAF-AH inhibitors |
Storage : | -20°C |
Size : | 100 assays |
Products Types
◆ Assay kits | ||
Kit-0644 | PAF Acetylhydrolase Activity Colorimetric Assay Kit | +Inquiry |
Kit-0645 | PAF Acetylhydrolase Assay Kit | +Inquiry |
Kit-0646 | PAF Acetylhydrolase Inhibitor Screening Assay Kit | +Inquiry |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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