|Product Overview:||Pyruvate is a key intermediate in cellular metabolic pathways. Pyruvate can be converted to carbohydrates via gluconeogenesis, to fatty acids or energy through acetyl-CoA, to the amino acid alanine and to ethanol. Abnormal levels of pyruvate have been linked to liver diseases and metabolic disorders. Simple, direct and automation-ready procedures for measuring pyruvate concentrations find wide applications in research and drug discovery. pyruvate assay uses a single Working Reagent that combines pyruvate oxidase and hydrogen peroxide determination in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to pyruvate concentration in the sample.|
|Detection method:||OD570nm, or FL530/585nm|
|Compatible Sample Types:||Biological|
|Features & Benefits:||Sensitive and accurate. Use as little as 10 μL samples. Linear detection range in 96-well plate: 2 to 500 μM (17 μg/dL to 4. 4 mg/dL) pyruvate for colorimetric assays and 0.2 to 50 μM for fluorimetric assays.
Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature, compatible for HTS assays.
Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability.
|Assay time:||20 min|
|Sensitivity:||OD, FL: 2, 0.2 μM|