| Cat.No.: | Kit-2514 |
| Product Overview: | Pyruvate is a key intermediate in cellular metabolic pathways. Pyruvate can be converted to carbohydrates via gluconeogenesis, to fatty acids or energy through acetyl-CoA, to the amino acid alanine and to ethanol. Abnormal levels of pyruvate have been linked to liver diseases and metabolic disorders. Simple, direct and automation-ready procedures for measuring pyruvate concentrations find wide applications in research and drug discovery. pyruvate assay uses a single Working Reagent that combines pyruvate oxidase and hydrogen peroxide determination in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to pyruvate concentration in the sample. |
| Storage: | -20ºC |
| Shipping: | On Ice |
| Size: | 100 tests |
| Detection method: | OD570nm, or FL530/585nm |
| Compatible Sample Types: | Biological |
| Features & Benefits: | Sensitive and accurate. Use as little as 10 μL samples. Linear detection range in 96-well plate: 2 to 500 μM (17 μg/dL to 4. 4 mg/dL) pyruvate for colorimetric assays and 0.2 to 50 μM for fluorimetric assays. Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature, compatible for HTS assays. Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability. |
| Assay time: | 20 min |
| Sensitivity: | OD, FL: 2, 0.2 μM |
Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.
Download Datasheet
See All Pyruvate Related Kits 
Download Datasheet