Recombinan Human EGLN1 protein, FLAG-tagged


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Recombinan Human EGLN1 protein, FLAG-tagged

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Cat.No. : EGLN1-001H
Product Overview : Recombinant human PHD2 (EGLN1) protein was expressed in a baculovirus expression system as the full length protein (accession number NP_071334.1) with an N-terminal FLAG tag.
Description : The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3).
Source : Insect cells
Species : Human
Tag : FLAG
Molecular Mass : 47 kDa
Applications : This protein is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
Storage : Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Storage Buffer : Recombinant PHD2 / EGLN1 protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5 mM TCEP.
10% SDS-PAGE Coomassie staining :

MALDI-TOF for PHD2 / EGLN1 protein :

3 µM HIF1A peptide was incubated with 300 nM PHD2 protein in 30 µl reaction system for 2 hours at 30 centigrade. The reaction product was detected by MALDI-TOF. Single 3 µM HIF1-α peptide was used as negative control.

Gene Name : EGLN1 egl-9 family hypoxia inducible factor 1 [ Homo sapiens (human) ]
Official Symbol : EGLN1
Synonyms : EGLN1; egl-9 family hypoxia inducible factor 1; HPH2; PHD2; SM20; ECYT3; HALAH; HPH-2; HIFPH2; ZMYND6; C1orf12; HIF-PH2; egl nine homolog 1; HIF-prolyl hydroxylase 2; egl nine-like protein 1; hypoxia-inducible factor prolyl hydroxylase 2; prolyl hydroxylase domain-containing protein 2; zinc finger MYND domain-containing protein 6; EC
Gene ID : 54583
mRNA Refseq : NM_022051
Protein Refseq : NP_071334
MIM : 606425
UniProt ID : Q9GZT9
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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