Recombinant Human EGLN1 protein

• Cat.No.: EGLN1-001H
• Product Overview: Recombinant human PHD2 (EGLN1) protein was expressed in a baculovirus expression system as the full length protein (accession number NP_071334.1) with an N-terminal FLAG tag.

Specification

• Description: The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3).
• Source: Insect cells
• Species: Human
• Tag: FLAG
• Molecular Mass: 47 kDa
• Applications: This protein is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Storage Buffer: Recombinant PHD2 / EGLN1 protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5 mM TCEP.

Gene Information

• Gene Name: EGLN1 egl-9 family hypoxia inducible factor 1 [ Homo sapiens (human) ]
• Official Symbol: EGLN1
• Synonyms: EGLN1; egl-9 family hypoxia inducible factor 1; HPH2; PHD2; SM20; ECYT3; HALAH; HPH-2; HIFPH2; ZMYND6; C1orf12; HIF-PH2; egl nine homolog 1; HIF-prolyl hydroxylase 2; egl nine-like protein 1; hypoxia-inducible factor prolyl hydroxylase 2; prolyl hydroxylase domain-containing protein 2; zinc finger MYND domain-containing protein 6; EC 1.14.11.29
• Gene ID: 54583
• mRNA Refseq: NM_022051
• Protein Refseq: NP_071334
• MIM: 606425
• UniProt ID: Q9GZT9