Recombinant 2019-nCoV NSP8 Protein, His-tagged

• Cat.No.: NSP8-08C
• Product Overview: Recombinant full-length 2019-nCoV NSP8 was expressed in E. coli using a C-terminal His tag.

Specification

• Description: Novel coronavirus SARS-CoV-2 has caused the pandemic of the respiratory diseases (COVID-19) around the world in 2020. Coronavirus RNA genome encodes a complex replication machinery consisting of 16 viral non-structural proteins (NSPs). NSP7 and NSP8 stabilize regions of NSP12 (RNA-dependent RNA polymerase; RdRp) involved in RNA binding and are essential for a highly active NSP12 polymerase complex. NSP7 has been suggested to confer RNA-binding properties to the trimeric polymerase complex. NSP8 has been shown to act as an oligo(U)-templated polyadenylyltransferase and a robust (mono/oligo) adenylate transferase. The NSP7/NSP8/NSP12 polymerase complex associates with NSP14 which is required to safeguard coronavirus replication fidelity
• Source: E. coli
• Species: 2019-nCoV
• Tag: His
• Molecular Mass: 22 kDa
• Purity: > 90%
• Applications: ELISA
• Stability: One year at -70 centigrade from date of shipment.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.2 μg/μL
• Storage Buffer: Recombinant protein stored in 20 mM Tris, pH7.5, 300mM NaCl, 200mM imidazole, 1 mM DTT, 10% glycerol.
• Shipping: Dry ice

Gene Information

• Gene Name: ORF1ab ORF1a polyprotein;ORF1ab polyprotein [ Severe acute respiratory syndrome coronavirus 2 ]
• Official Symbol: ORF1ab
• Synonyms: ORF1ab; ORF1a polyprotein;ORF1ab polyprotein; ORF1a polyprotein;ORF1ab polyprotein; leader protein; nsp2; nsp3; nsp4; 3C-like proteinase; nsp6; nsp7; nsp8; nsp9; nsp10; RNA-dependent RNA polymerase; helicase; 3'-to-5' exonuclease; endoRNAse; 2'-O-ribose methyltransferase; leader protein; nsp2; nsp3; nsp4; 3C-like proteinase; nsp6; nsp7; nsp8; nsp9; nsp10; nsp11; pp1ab; translated by -1 ribosomal frameshift; pp1a
• Gene ID: 43740578
• Protein Refseq: YP_009725304