Recombinant COVID-19 NSP8 protein, His-tagged NSP8-198V

Recombinant COVID-19 NSP8 protein, His-tagged

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Recombinant COVID-19 NSP8 protein, His-tagged

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Cat.No. : NSP8-198V
Product Overview : Recombinant COVID-19 NSP8 protein (Ala1-Gln198) was fused to his-tag at the N-terminus and expressed in E.coli.
Description : NSP8, forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers. To ultimately combat the emerging COVID-19 pandemic, it is desired to develop an effective and safe vaccine against this highly contagious disease caused by the SARS-CoV-2 coronavirus. By investigating the entire proteome of COVID-19, six proteins, including the S protein and five non-structural proteins (nsp3, 3CL-pro, and nsp8-10) were predicted to be adhesins, which are crucial to the viral adhering and host invasion. The S, nsp3, and nsp8 proteins were also predicted by Vaxign-ML to induce high protective antigenicity.
Source : E.coli
Species : COVID-19
Tag : His
Predicted N Terminal : Met
Form : Delivered as bulk protein in a 0.2 μm filtered solution of 20 mM Tris, 250 mM NaCl, pH8.5 with glycerol as protectant.
Molecular Mass : The protein has a calculated MW of 23.9 kDa. The protein migrates as 22-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Ala1-Gln198
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
The product MUST be stored at -70°C or lower upon receipt;
-70°C for 3 months under sterile conditions.
Shipping : This product is supplied as sterile liquid solution and shipped frozen with dry ice, please inquire the shipping cost.
SDS-PAGE of NSP8-198V :

COVID-19 NSP8, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Gene Name : ORF1ab
Official Symbol : ORF1ab
Synonyms : ORF1a polyprotein
Gene ID : 43740578
Protein Refseq : YP_009725304.1
UniProt ID : P0DTD1

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