Recombinant COVID-19 NSP8 protein, His-tagged

• Cat.No.: NSP8-198V
• Product Overview: Recombinant COVID-19 NSP8 protein (Ala1-Gln198) was fused to his-tag at the N-terminus and expressed in E.coli.

Specification

• Description: NSP8, forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers. To ultimately combat the emerging COVID-19 pandemic, it is desired to develop an effective and safe vaccine against this highly contagious disease caused by the SARS-CoV-2 coronavirus. By investigating the entire proteome of COVID-19, six proteins, including the S protein and five non-structural proteins (nsp3, 3CL-pro, and nsp8-10) were predicted to be adhesins, which are crucial to the viral adhering and host invasion. The S, nsp3, and nsp8 proteins were also predicted by Vaxign-ML to induce high protective antigenicity.
• Source: E.coli
• Species: COVID-19
• Tag: His
• Predicted N Terminal: Met
• Form: Delivered as bulk protein in a 0.2 μm filtered solution of 20 mM Tris, 250 mM NaCl, pH8.5 with glycerol as protectant.
• Molecular Mass: The protein has a calculated MW of 23.9 kDa. The protein migrates as 22-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Ala1-Gln198
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  The product MUST be stored at -70°C or lower upon receipt;
  -70°C for 3 months under sterile conditions.
• Shipping: This product is supplied as sterile liquid solution and shipped frozen with dry ice, please inquire the shipping cost.

Gene Information

• Gene Name: ORF1ab
• Official Symbol: ORF1ab
• Synonyms: ORF1a polyprotein
• Gene ID: 43740578
• Protein Refseq: YP_009725304.1
• UniProt ID: P0DTD1