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Active Recombinant 2019-nCoV Spike RBD(F490L) Protein, His-tagged

Cat.No. : Spike-367V
Product Overview : Recombinant 2019-nCoV Spike RBD(F490L) Protein (YP_009724390.1) (Arg 319 - Lys 537(F490L)) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.
  • Specification
  • Gene Information
  • Related Products
Source : HEK293
Species : 2019-nCoV
Tag : His
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized SARS-CoV-2 S protein RBD (F490L), His Tag at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag with a linear range of 0.1-2 ng/mL.
Molecular Mass : The protein has a calculated MW of 26.6 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein Length : Arg 319 - Lys 537
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
Gene Name : S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
Official Symbol : Spike
Synonyms : Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1; COVID-19
Gene ID : 43740568
Protein Refseq : YP_009724390.1
UniProt ID : P0DTC2

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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