Recombinant 2019-nCoV Spike S1 (T19R, G142D, E156G, 157-158 deletion, L452R, T478K, D614G, P681R) Protein, His-tagged

• Cat.No.: Spike-470V
• Product Overview: Recombinant 2019-nCoV Spike S1 (T19R, G142D, E156G, 157-158 deletion, L452R, T478K, D614G, P681R) Protein(YP_009724390.1)(Met1-Arg685), fused to His tag, was expressed in HEK293.

Specification

• Species: Sars-Cov-2
• Source: HEK293
• Tag: His
• Protein Length: Met1-Arg685
• Form: Lyophilized from sterile PBS, pH 7.4.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant SARS-CoV-2 Spike S1 consists of 679 amino acids and predicts a molecular mass of 76.27 kDa. As a result of glycosylation, it migrates as an approximately 95.12 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
• Official Symbol: S
• Synonyms: Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1; COVID-19
• Gene ID: 43740568
• Protein Refseq: YP_009724390.1
• UniProt ID: P0DTC2