Recombinant Active Human CDK12/Cyclin K Complex Protein, GST-tagged

• Cat.No.: CDK12-07H
• Product Overview: Recombinant human CDK12 (658-end) /Cyclin K complex was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.

Specification

• Description: Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors.
• Source: Insect cell
• Species: Human
• Tag: GST
• Molecular Mass: CDK12: Approx 135 kDa
  CyclinK: Approx 67 kDa
• Purity: > 85%
• Stability: One year at -70 centigrade from data of shipment.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.05 μg/μL
• Storage Buffer: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 25% glycerol.
• Shipping: Dry ice

Gene Information

• Gene Name: CDK12 cyclin dependent kinase 12 [ Homo sapiens (human) ]
• Official Symbol: CDK12
• Synonyms: CDK12; cyclin dependent kinase 12; cyclin-dependent kinase 12; CDC2-related protein kinase 7; Cdc2-related kinase, arginine/serine-rich; cell division cycle 2-related protein kinase 7; cell division protein kinase 12; EC 2.7.11.22; EC 2.7.11.23
• Gene ID: 51755
• mRNA Refseq: NM_015083
• Protein Refseq: NP_055898
• MIM: 615514
• UniProt ID: Q9NYV4