Recombinant Active Human IL22 Protein, His-tagged(C-ter)

• Cat.No.: IL22-177H
• Product Overview: Recombinant Active Human IL22 Protein with His tag (C-ter) was expressed in E. coli.

Specification

• Description: This gene is a member of the IL10 family of cytokines that mediate cellular inflammatory responses. The encoded protein functions in antimicrobial defense at mucosal surfaces and in tissue repair. This protein also has pro-inflammatory properties and plays a role in in the pathogenesis of several intestinal diseases. The encoded protein is a crucial cytokine that regulates host immunity in infectious diseases, including COVID-19 (disease caused by SARS-CoV-2).
• Source: E. coli
• Species: Human
• Tag: His
• Form: Powder
• Bio-activity: Determined by its ability to induce proliferation in A549 cells. The ED50 for this effect is < 0.5 ng/mL.
• AA Sequence: MAPISSHCRLDKSNFQQPYITNRTF MLAKEASLADNNTDVRLIGEKLFHG VSMSERCYLMKQVLNFTLEEVLFPQ SDRFQPYMQEVVPFLARLSNRLSTC HIEGDDLHIQRNVQKLKDTVKKLGE SGEIKAIGELDLLFMSLRNACI
• Endotoxin: Endotoxin level is less than 0.1 EU/μg of the protein, as determined by the LAL test.
• Purity: > 98% (by SDS-PAGE)
• Applications: SDS-PAGE
• Notes: For laboratory research only, not for drug, diagnostic or other use.
• Storage: Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
• Storage Buffer: PBS (pH 8.0)
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.

Gene Information

• Gene Name: IL22 interleukin 22 [ Homo sapiens ]
• Official Symbol: IL22
• Synonyms: IL22; interleukin 22; interleukin-22; IL 10 related T cell derived inducible factor; IL 21; IL 22; IL D110; IL TIF; ILTIF; MGC79382; MGC79384; TIFa; TIFIL 23; zcyto18; cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL-10-related T-cell-derived-inducible factor; IL-21; IL-22; IL-TIF; IL-D110; TIFIL-23
• Gene ID: 50616
• mRNA Refseq: NM_020525
• Protein Refseq: NP_065386
• MIM: 605330
• UniProt ID: Q9GZX6

Reviews

04/07/2021

High specificity.

03/29/2021

Good for Th17 differentiation.

07/07/2016

Effective in gut repair assays.

Q&As

What is IL22's role in gut epithelial repair?

IL22 enhances gut epithelial repair mechanisms.

What are the downstream targets of IL22 signaling?

IL22 activates STAT3 to modulate downstream targets.

How does IL22 interact with other cytokines in inflammation?

IL22 interacts synergistically with IL-17 during inflammation.

Is IL22 involved in the pathogenesis of autoimmune diseases?

IL22 is involved in the pathogenesis of autoimmune diseases like Crohn's.

Does IL22 have a role in metabolic syndrome?

IL22 is implicated in metabolic syndrome by affecting insulin sensitivity.

How is IL22 expression regulated in psoriasis?

IL22 expression is upregulated in psoriasis.

Does IL22 expression change during viral infections?

IL22 expression is upregulated during viral infections like Hepatitis C.

Does IL22 contribute to anti-tumor immunity?

IL22 contributes to anti-tumor immunity by activating NK cells.

How does IL22 modulate T-cell differentiation?

IL22 promotes Th22 cell differentiation.

What impact do IL22 polymorphisms have on its function?

IL22 polymorphisms affect its binding affinity and signaling.