Recombinant Active Lachnospiraceae Cas12 (LbCpf1) Protein, His-tagged

• Cat.No.: Cas12 (LbCpf1)-17L
• Product Overview: Recombinant Lachnospiraceae Cas12 (LbCpf1) (1-1227) was expressed in E. coli with a C-terminal His tag.

Specification

• Species: Lachnospiraceae
• Source: E.coli
• Tag: His
• Protein Length: 1-1227 a.a.
• Description: The severe acute respiratory syndrome related novel coronavirus SARS-CoV-2 has caused the pandemic of the respiratory diseases (COVID-19) around the world in 2020. Developing rapid and sensitive diagnostic tools is key for successful patient management and curbing the disease spread. Several CRISPR-Cas12 based SARS-CoV-2 detection assays have been developed. Comparing to conventional RT-PCR based tests that are hindered by the need for expensive lab instrumentation, Cas12 based tests are ultrasensitive, rapid and in low-cost POCT formats.
• Molecular Mass: Approx 130 kDa (calculated ~140 kDa)
• Purity: >85%
• Stability: One year at -70 centigrade from date of shipment.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.05 μg/μL
• Storage Buffer: Recombinant protein stored in 50 mM sodium phosphate, pH 7.5, 300mM NaCl, 150mM imidazole, 10% glycerol, 1 mM DTT.
• Shipping: Dry ice

Gene Information

• Synonyms: Cas12 (LbCpf1)
• mRNA Refseq: ATB19154