"GFP" Related Products

Recombinant Aequorea victoria GFP Protein, His-tagged

Cat.No.: GFP-1031A
Product Overview: Recombinant Aequorea victoria GFP (AAB65663) (Met1-Leu238, except for the point mutations of intellectual property?:F64L, S65T, R80K, S99F, T153M, I167V, H231L) was expressed, with a polyhistide tag at the C-terminus (Patent 201410599023.9).
Source: E. coli
Species: Aequorea victoria
Tag: His
Predicted N Terminal: Met
Form: Lyophilized from sterile PBS, pH 7.51, 5%~8% trehalose and mannitol.
Bio-activity: GFPSparkTM expression vector transfected 293H cells transiently. After 48h, the strong green fluorescent signals can be detected under the excitation channel of 455~495 nm.
Molecular Mass: The recombinant Aequorea victoria GFPSparkTM consisting of 245 amino acids and has a calculated molecular mass of 27.7 kDa.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >97 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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