Recombinant Aequorea victoria GFP Protein, His-tagged


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Recombinant Aequorea victoria GFP Protein, His-tagged

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Cat.No. : GFP-1031A
Product Overview : Recombinant Aequorea victoria GFP (AAB65663) (Met1-Leu238, except for the point mutations of intellectual property?:F64L, S65T, R80K, S99F, T153M, I167V, H231L) was expressed, with a polyhistide tag at the C-terminus (Patent 201410599023.9).
Source : E. coli
Species : Aequorea victoria
Tag : His
Predicted N Terminal : Met
Form : Lyophilized from sterile PBS, pH 7.51, 5%~8% trehalose and mannitol.
Bio-activity : GFPSparkTM expression vector transfected 293H cells transiently. After 48h, the strong green fluorescent signals can be detected under the excitation channel of 455~495 nm.
Molecular Mass : The recombinant Aequorea victoria GFPSparkTM consisting of 245 amino acids and has a calculated molecular mass of 27.7 kDa.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >97 % as determined by SDS-PAGE.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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