Recombinant African clawed frog pparg protein, His-tagged

• Cat.No.: pparg-5353A
• Product Overview: Recombinant African clawed frog pparg protein(P37234)(1-477aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Source: E.coli
• Species: African clawed frog
• Tag: N-His
• Protein length: 1-477aa
• Form: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Molecular Mass: 58.1 kDa
• AASequence: MVDTEMPFWSNLNFGMNSMDMSALE DHCQPYDIKPFTTVDFSSINSHYDD ILDEKTFLCRNDQSPIDYKYDLKLQ ECQSSIKLEPPSPPYFSDKPQCSKA FEDTPNSFIAIECRVCGDKASGFHY GVHACEGCKGFFRRTIRLKLIYERC DLNCRIHKKSRNKCQFCRFQKCLAV GMSHNAIRFGRMPQAEKEKLLAEIS SDIDQLNPESADQRVLAKHLYDSYV KSFPLTKAKAPGHPDGQSHRQNSRG YTRHELADDGGGSDQGAVREPRAEQ GGGDSNLPALSVALRGGVREITEFA KNIPGFVSLDLNDQVTLLKYGVHEI IFTMLASLMNKDGVLVAEGQGFMTR EFLKSLRKPFSDFMEPKFEFAIRFN SLELDDSDLAIFVAVIILSGDRPGL LNVKPIEDIQDSLLQALELQLKLNH PDSAQLFAKLLQKMTDLRQVVTEHV QLLQLIKKTEADMCLHPLLQEIYKD LY
• Purity: Greater than 85% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C.

Reviews

09/15/2020

It was reassuring to use a product that adhered to green chemistry principles, reducing our impact on the environment.

06/03/2017

The company's dedication to environmental sustainability reflected positively on the product and our research initiatives.

05/04/2017

We appreciated the option to request modifications or custom formulations to meet our project's requirements.

Q&As

How many isoforms of PPARG are there, and what are their weights?

PPARG has two isoforms, PPARG1 (57 kDa) and PPARG2, a splice variant with an additional 30 amino acids at the N-terminus.

What is the molecular weight of PPARG?

PPARG has two isoforms with molecular weights of 57 kDa and 54 kDa. Modified PPARG is about 67 kDa.

What does PPARG stand for?

PPARG stands for Peroxisome Proliferator-Activated Receptor Gamma.

Is there a mitochondria-localized form of PPARG?

Yes, experimental data suggests a 45 kDa protein immunologically related to PPARG, called mt-PPAR, is located in mitochondria. Its molecular weight is less than PPARG2.

What is the role of PPARG in cellular function?

PPARG is a ligand-activated transcription factor that plays a crucial role in regulating lipid homeostasis, adipogenesis, insulin resistance, and organ development.

What are the known diseases associated with PPARG defects?

Defects in PPARG are associated with familial partial lipodystrophy type 3 (FPLD3), susceptibility to obesity, type 2 insulin-resistant diabetes, hypertension, and colon cancer.

Where is PPARG mainly localized?

PPARG is mainly localized in the nucleus, although it can also be detected in the cytoplasm, exhibiting extra-nuclear/non-genomic actions in certain contexts.