Recombinant B. burgdorferi DbpB Protein, MBP-tagged

• Cat.No.: DbpB-04B
• Product Overview: Recombinant Borrelia burgdorferi DbpB protein produced in E. coli. Protein contains a MBP-tag and is visible on SDS-PAGE at 60.3 kDa for DbpB-MBP (17.9 kDa for DbpB and 42.4 kDa for MBP)

Specification

• Description: Decorin Binding Protein B (DbpB) is encoded by the spirochete B. burgdorferi, which is carried by Ixodes ticks. Strain B31 is the type strain (ATCC 35210) for this organism and was derived by limited dilutional cloning from the original Lyme-disease tick isolate obtained by A. Barbour. It should be noted that other microbial DbpA’s, such as E. coli (ATP-dependent RNA helicase DbpA), are significantly different to Borrelia DbpA. B. burgdorferi sensu stricto has a linear plasmid (lp54) which carries a two-gene operon encoding two surface lipoproteins, DbpA and DbpB, both of which bind decorin.
• Source: E. coli
• Species: Borreliella burgdorferi B31
• Tag: MBP
• Form: Liquid, 0.2 μm filter sterilised.
• Molecular Mass: 60.3 kDa for DbpB-MBP (17.9 kDa for DbpB and 42.4 kDa for MBP)
• Purity: > 90%
• Storage: At -20 centigrade or below.
  Can be frozen, but avoid multiple freeze/thaw cycles
• Concentration: 1.0 mg/mL
• Storage Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide.

Gene Information

• Gene Name: dbpB decorin-binding protein DbpB [ Borreliella burgdorferi B31 ]
• Official Symbol: dbpB
• Synonyms: dbpB; decorin-binding protein DbpB
• Gene ID: 1194341
• Protein Refseq: WP_010890381