Recombinant B. canis Merozoite Surface Antigen, His tagged, Biotinylated

• Cat.No.: MSA1-078B
• Product Overview: Recombinant antigen formed by the fragment containing a 1 to 303 of BcMSA1 for B. canis and fused to a His-tag in its C-terminus and monobiotinylated in vivo.

Specification

• Species: B. canis
• Source: P.pastoris
• Tag: His
• Protein Length: 1-303aa
• Description: Merozoite surface antigen (BcMSA1) was described as a potential candidate for babesiosis serodiagnosis, evaluated by ELISA.
• Tag: C-His
• Conjugation/Label: Biotin
• Molecular Mass: SDS-PAGE analysis determines that the protein band is between the molecular markers of 116 kDa and 66.2 kDa due to the glycosylation pattern, while relative molecular mass, calculated from amino acid sequence and without glycosylation, is 43,031.64 Da.
• Purity: > 95% by observation on SDS-PAGE electrophoresis
• Applications: WB, DB, CE, NP, PO
• Storage: Upon arrival, it should be aliquoted to avoid repeated freezing and thawing cycles and stored at -20 to -80 centigrade. In order to defrost the protein, maintain the aliquot at 25 centigrade without shaking to avoid aggregation.
• Storage Buffer: 20 mM phosphate buffer pH 8 and 0.15 M NaCl
• Concentration: 1.11 mg/mL
• Shipping: Protein is shipped with dry ice.
• Reference: 1. Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.
• Conjugation: Biotin