Recombinant B. canis secreted antigen 1, His tagged

• Cat.No.: SA1-080B
• Product Overview: Recombinant antigen formed by the mature protein BcSA1 for B. canis and fused to a His-tag in its N-terminus.

Specification

• Species: B. canis
• Source: E.coli
• Tag: His
• Description: Recombinant secreted antigen 1 (BcSA1) was described as a potential candidate for babesiosis serodiagnosis, evaluated by ELISA.
• Tag: N-His
• Molecular Mass: Determined by SDS-PAGE, the protein band is between molecular markers of 45-66.2 kDa, while relative molecular mass calculated from amino acid sequence is 47,711.61.
• Purity: > 95% by observation on SDS-PAGE electrophoresis
• Applications: WB, DB, IE, DE, CLIA, LF
• Storage: Upon arrival, it should be aliquoted to avoid repeated freezing and thawing cycles and stored at -20 to -80 centigrade. In order to defrost the protein, maintain the aliquot at 25 centigrade without shaking to avoid aggregation.
• Storage Buffer: 20 mM MOMPS pH 7, 0.15 M NaCl and 0.1% polyoxyethylene (10) tridecyl ether
• Concentration: 1.57 mg/mL
• Shipping: Protein is shipped with dry ice.
• Reference: 1. Zhou1, M., Cao, S., Luo, Y., Liu, M., Wang, G., Moumouni, P.F.A., Jirapattharasate, C., Iguchi, A., Vudriko, P., Terkawi, M. A., Löwenstein, M., Kern, A., Nishikawa, Y., Suzuki, H., Igarashi, I. and X. Xuan. Molecular identification and antigenic characterization of a merozoite surface antigen and a secreted antigen of Babesia canis (BcMSA1 and BcSA1). Parasites & Vectors (2016) 9:257.
  2. Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.