Recombinant Bacillus subtilis ACCB protein, His-tagged

• Cat.No.: ACCB-1599B
• Product Overview: Recombinant Bacillus subtilis ACCB full length or partial length protein was expressed.

Specification

• Source: E. coli or Yeast
• Species: Bacillus subtilis
• Tag: His
• Form: Liquid or lyophilized powder
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 80% by SDS-PAGE
• Notes: This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
• Storage: Store it at +4 ºC for short term. For long term storage, store it at -20 ºC~-80 ºC.
• Storage buffer: PBS buffer

Gene Information

• Gene Name: accB acetyl-CoA carboxylase biotin carboxyl carrier protein subunit [ Bacillus subtilis subsp. subtilis str. 168 ]
• Official Symbol: ACCB
• Synonyms: ACCB; acetyl-CoA carboxylase biotin carboxyl carrier protein subunit
• Gene ID: 938587
• UniProt ID: P49786

Reviews

09/14/2022

The Recombinant ACCB Protein from Creative BioMart exceeded my expectations. It was easy to use and produced excellent results in my assays.

01/12/2022

I am very happy with my purchase of the recombinant ACCB protein from Creative BioMart. The protein was shipped quickly and arrived in perfect condition.

08/22/2021

I am very satisfied with the quality and performance of the Recombinant ACCB Protein (ACCB-1599B). It is a reliable product that I will continue to use in my research.

Q&As

Does your recombinant Bacillus subtilis ACCB protein with another tag?

We can help you choose the suitable expression system and tag that meets your specific needs.

How can the role of ACCB in cancer be further elucidated?

Additional studies are needed to understand the regulation of ACCB expression in different cancer types, as well as the downstream effects of ACCB inhibition on cancer metabolism and signaling pathways. Further investigation into ACCB isoform specificity and the role of ACCB mutations in cancer could also help clarify the role of ACCB as a therapeutic target.

How can recombinant ACCB protein be used in computational modeling of cancer metabolism?

It can be used in combination with other biochemical and biophysical data to generate computational models of cancer metabolism and predict the effects of genetic or pharmacological perturbations on this system. These models can be used to identify new targets for cancer therapy or to optimize existing therapies.

What is the recommended concentration of recombinant ACCB protein for use in research experiments, and what is the optimal buffer for diluting the protein?

The recommended concentration of recombinant ACCB protein for use in research experiments may vary depending on the specific application. However, a typical starting concentration is 0.1-1 mg/ml. The optimal buffer for diluting the protein may also vary depending on the experiment, but a common choice is phosphate-buffered saline (PBS).

How is ACCB expression regulated in cancer cells?

ACCB expression is upregulated in many types of cancer cells, and several signaling pathways have been identified that regulate ACCB expression, including mTORC1 and AMPK pathways.

What is the recommended method for storing and handling recombinant ACCB protein to maintain its stability and activity?

To maintain the stability and activity of recombinant ACCB protein, it is recommended to store the protein below –20°C and away from repeated freeze-thaw cycles. It is also recommended to avoid exposing the protein to high temperatures, mechanical agitation, or prolonged exposure to light or air.

What are the challenges in developing ACCB inhibitors as cancer therapeutics?

One challenge is the potential for off-target effects, as ACCB is also involved in other metabolic pathways. Another challenge is the development of drug resistance, as cancer cells can acquire mutations that bypass the inhibition of ACCB.

How can recombinant ACCB protein be used in drug discovery?

Recombinant ACCB protein can be used as a target for high-throughput screening assays to identify small molecules or other compounds that inhibit ACCB activity. These inhibitors could then be further developed into potential drugs for cancer or metabolic disorders.

Can your recombinant ACCB proteins be used for WB?

Yes, it is suitable for various applications based on antigen-antibody reactivity.

May I receive a discount on recombinant ACCB protein as a first-time buyer?

All first-time customers are offered coupons. We appreciate your inquiry and look forward to receiving your subsequent order.

What is the endotoxin level of the recombinant ACCB protein product, and what method is used to measure it?

The endotoxin level of the recombinant ACCB protein offered by Creative BioMart is typically less than 1.0 EU/μg as determined by the Limulus Amebocyte Lysate (LAL) assay. This assay is a widely accepted method for measuring the presence of bacterial endotoxins in protein samples. In addition, we support the production of recombinant proteins with different endotoxin levels, and you can choose a lower endotoxin level when submitting the inquiry form.

How can the activity and specificity of recombinant ACCB protein be assessed?

Recombinant ACCB protein activity can be assessed using enzymatic assays that measure the carboxylation of acetyl-CoA to malonyl-CoA. Its specificity can be evaluated through the use of selective inhibitors or mutagenesis studies that alter the active site of the protein.

How can recombinant ACCB protein be used to study cancer metabolism?

It can be used as a tool to study the regulation of fatty acid biosynthesis in cancer cells and to evaluate the effects of inhibitors or other compounds on this pathway. Additionally, recombinant ACCB protein can be used to identify downstream signaling pathways that are affected by ACCB inhibition.

Does Creative BioMart offer custom services for recombinant ACCB protein, such as purification or labeling?

Yes, we offer a variety of custom services.