| Description : |
GCP-2 (granulocyte chemotactic protein-2) is a CXC chemokine. Among human CXC chemokines, GCP-2 is most closely related to ENA-78. The structure and sequence of the genes for human GCP-2 and ENA-78 also exhibit close similarity, suggesting the two genes may have originated from a recent gene duplication. LIX (LPS-induced CXC chemokine) was initially cloned as a gene induced by LPS in mouse fibroblasts. The mouse protein designated GCP-2, because of its amino acid sequence similarity (60%) to human GCP-2, is identical to the LIX protein sequence. |
| Source : |
E.coli |
| Species : |
Bovine |
| Form : |
Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, 500 mM NaCl, pH 7.0. |
| Bio-activity : |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 10-50 ng/ml. |
| Molecular Mass : |
Approximately 8.0 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids. |
| Protein length : |
76 |
| AA Sequence : |
GPVAAVVRELRCVCLTTTPGIHPKT VSDLQVIAAGPQCSKVEVIATLKNG REVCLDPEAPLIKKIVQKILDSGKN N |
| Endotoxin : |
Less than 0.1 EU/µg of rBoGCP-2/CXCL6 as determined by LAL method. |
| Purity : |
>97% by SDS-PAGE and HPLC analyses. |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
