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Recombinant Bovine TMPRSS15 protein, His-tagged

Cat.No. : TMPRSS15-173B
Product Overview : Recombinant Bovine TMPRSS15 protein, fused to His-tag at C-terminus, was expressed in Escherichia coli.
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  • Gene Information
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Description : Enterokinase (EK) is an amino protease existing in duodenum of mammal and is involved in digestion. It consists of a disulfide-linked 82–140 kDa heavy chain which anchors enterokinase in the intestinal brush border membrane and a 35–62 kDa light chain which contains the catalytic subunit. Additionally, both of the chains are derived from a single precursor that is cleaved by a trypsin-like protease. EK can specially recognize the amino acid sequence DDDDK, and digest the peptide bond after the lysine residue.rEK was report to be more effective than nature EK in cleaving recombinant proteins. Furthermore, the light chain possesses the whole enzyme activity of EK. rBoEK has the highest activity than EK of other species and is used wildly in biochemical applications. rBoEK with 6 × His-tag binds with Ni2+ affinity chromatography and was designed for removing from digestion system.
Source : E.coli
Species : Bovine
Tag : His
Form : 50 mM Tris-HCl, pH 8.0, 0.5 M NaCl and 50 % glycerol.
Molecular Mass : Approximately 28 kDa, a single non-glycosylated polypeptide chain containing 241 amino acids,with 6 × His at C-terminus.
Protein length : 241
Endotoxin : Less than 1 EU/µg of rBoEKL, His as determined by LAL method.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 centigrade as supplied. 3 months, -20 to -70 centigrade under sterile conditions after opening.
Gene Name : TMPRSS15
Official Symbol : TMPRSS15
Synonyms : Enterokinase, Serine Protease 7, Transmembrane Protease Serine 15
Gene ID : 282009
mRNA Refseq : NM_174439.2
Protein Refseq : NP_776864.1
UniProt ID : P98072

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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