Recombinant Brevibacillus sp Cas12b Protein, His-tagged
Cat.No. : | Cas12b-04B |
Product Overview : | Recombinant Brevibacillus sp BrCas12b (1-1090) was expressed in E. coli cells with a C-terminal His tag. |
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Description : | CRISPR (clustered regularly interspaced short palindromic repeat) and CRISPR-associated (Cas) proteins constitute the adaptive immune system in bacteria. This system has been redesigned to create an exemplary genome editing tool for RNA-based therapeutics development. Cas12 and Cas13 have collateral nucleotide cleavage activities and are widely used in CRISPR-based diagnostic platforms. BrCas12b from Brevibacillus shows robust trans-cleavage activity at ideal LAMP (loop-mediated isothermal amplification) temperature. The thermal stability of BrCas12b enables one-pot target nucleic detection to minimize carryover contamination. |
Source : | E. coli |
Species : | Brevibacillus Sp |
Tag : | His |
Protein Length : | 1-1090 |
Stability : | One year at -70 centigrade from dare of shipment. |
Storage : | Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated handling and multiple freeze/thaw cycles. |
Concentration : | 0.23 μg/μL |
Storage Buffer : | 150mM imidazole, 50mM sodium phosphate, pH 7.5, 1mM DTT, 10% glycerol. |
Official Symbol : | Cas12b |
Synonyms : | c2c1; CRISPR-associated endonuclease C2c1; Cas12b |
Protein Refseq : | WP_165214399 |
Official Symbol : | Cas12b |
Synonyms : | c2c1; CRISPR-associated endonuclease C2c1; Cas12b |
Protein Refseq : | WP_165214399 |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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