Recombinant Candida albicans eno1 Antigen, His tagged

• Cat.No.: eno1-013C
• Product Overview: The Candida albicans enolase gene has been expressed as a recombinant antigen fused to a his-tag in its N-terminus. It is produced from the complete ORF of the 2-phospho-D-glyceratehydro-lyase.

Specification

• Species: Candida albicans
• Source: E.coli
• Tag: His
• Description: Enolase (2-phosphoD-glycerate hyidrolyase) is an important glycolytic enzyme located on the cell wall of Candida albicans. This is a termostable and proteinic antigen, produced by all candida species. It is a marker of deep tissular invasion, detectable even in absence of candidemia. It has been described as highly immunogenic.
• Tag: N-His
• Molecular Mass: Determined by SDS-PAGE, the protein band is between molecular markers of 66.2-45 kDa, while relative molecular mass calculated from amino acid sequence is 56,543.7 Da.
• Purity: > 95% by observation on SDS-PAGE electrophoresis
• Applications: WB, DB, IE, DE
• Storage: Upon arrival, it should be aliquoted to avoid repeated freezing and thawing cycles and stored at -20 to -80 centigrade. In order to defrost the protein, maintain the aliquot at 25 centigrade without shaking to avoid aggregation.
• Storage Buffer: 20 mM phosphate buffer pH 8, 1 M NaCl, 0.1% polyoxyethylene (10) tridecylether and 8 M urea
• Concentration: 1.28 mg/mL
• Shipping: Protein is shipped with dry ice.
• Reference: 1. Eroles, P., Sentandreu, M., Elorza, M.V. and Sentandreu, R. The highly immunogenic enolase and HSP70 are adventitious Candida albicans cell Wall proteins. 1997, Microbiology, 143:313–20.
  2. Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.