Active Recombinant Canine MET Protein, His-tagged

• Cat.No.: MET-1037C
• Product Overview: Recombinant extracellular domain of canine MET (NP_001002963.1) (Met 1-Leu 935) was expressed, with a polyhistidine tag at the N-terminus.

Specification

• Source: HEK293
• Species: Canine
• Tag: His
• Predicted N Terminal: Glu 25
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. 2. Immobilized human HGF at 10 μg/mL (100 μl/well) can bind canine c-MET, The EC50 of canine c-MET is 7 ng/mL. 3. Immobilized canine MET-His at 10 μg/ml (100 μl/well) can bind biotinylated human HG
• Molecular Mass: The recombinant canine c-Met is a disulfide-linked heterodimer composed of proteolytically cleaved α and β subunits. Each α and β subunit together consists of 922 amino acids and has a predicted molecular mass of 103 (α =33 + β =70) kDa. As a result of glycosylation, the apparent molecular mass of the canine c-Met is approximately 42-47 kDa and 85-95 kDa respectively in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: MET MET proto-oncogene, receptor tyrosine kinase [ Canis lupus familiaris ]
• Official Symbol: MET
• Synonyms: c-Met; HGF receptor; HGF/SF receptor; SF receptor; met proto-oncogene (hepatocyte growth factor receptor); met proto-oncogene tyrosine kinase; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met
• Gene ID: 403438
• mRNA Refseq: NM_001002963
• Protein Refseq: NP_001002963
• UniProt ID: Q75ZY9
• Chromosome Location: chromosome: 14
• Pathway: Adherens junction, organism-specific biosystem; Axon guidance, conserved biosystem; Central carbon metabolism in cancer, organism-specific biosystem
• Function: ATP binding; transmembrane receptor protein tyrosine kinase activity