"MET" Related Products

Expand
Expand

Recombinant Canine MET Protein, His-tagged

Cat.No.: MET-1037C
Product Overview: Recombinant extracellular domain of canine MET (NP_001002963.1) (Met 1-Leu 935) was expressed, with a polyhistidine tag at the N-terminus.
Source: HEK293
Species: Canine
Tag: His
Predicted N Terminal: Glu 25
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity: 1. Measured by its binding ability in a functional ELISA. 2. Immobilized human HGF at 10 μg/mL (100 μl/well) can bind canine c-MET, The EC50 of canine c-MET is 7 ng/mL. 3. Immobilized canine MET-His at 10 μg/ml (100 μl/well) can bind biotinylated human HG
Molecular Mass: The recombinant canine c-Met is a disulfide-linked heterodimer composed of proteolytically cleaved α and β subunits. Each α and β subunit together consists of 922 amino acids and has a predicted molecular mass of 103 (α =33 + β =70) kDa. As a result of glycosylation, the apparent molecular mass of the canine c-Met is approximately 42-47 kDa and 85-95 kDa respectively in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >95 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: MET MET proto-oncogene, receptor tyrosine kinase [ Canis lupus familiaris ]
Official Symbol: MET
Synonyms: c-Met; HGF receptor; HGF/SF receptor; SF receptor; met proto-oncogene (hepatocyte growth factor receptor); met proto-oncogene tyrosine kinase; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met
Gene ID: 403438
mRNA Refseq: NM_001002963
Protein Refseq: NP_001002963
MIM:
UniProt ID: Q75ZY9
Chromosome Location: chromosome: 14
Pathway: Adherens junction, organism-specific biosystem; Axon guidance, conserved biosystem; Central carbon metabolism in cancer, organism-specific biosystem
Function: ATP binding; transmembrane receptor protein tyrosine kinase activity

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information


Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.