Recombinant Clostridium Botulinum BOTB Protein (1-427 aa), His-tagged

• Cat.No.: BOTB-1573C
• Product Overview: Recombinant Clostridium Botulinum BOTB Protein (1-427 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Partial.

Specification

• Description: Botulinum toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that cleaves the '76-Gln-|-Phe-77' bond of synaptobrevin-2.
• Source: Yeast
• Species: Clostridium Botulinum
• Tag: His
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 51.3 kDa
• Protein length: 1-427 aa
• AA Sequence: MPVTINNFNYNDPIDNNNIIMMEPP FARGTGRYYKAFKITDRIWIIPERY TFGYKPEDFNKSSGIFNRDVCEYYD PDYLNTNDKKNIFLQTMIKLFNRIK SKPLGEKLLEMIINGIPYLGDRRVP LEEFNTNIASVTVNKLISNPGEVER KKGIFANLIIFGPGPVLNENETIDI GIQNHFASREGFGGIMQMKFCPEYV SVFNNVQENKGASIFNRRGYFSDPA LILMHELIHVLHGLYGIKVDDLPIV PNEKKFFMQSTDAIQAEELYTFGGQ DPSIITPSTDKSIYDKVLQNFRGIV DRLNKVLVCISDPNININIYKNKFK DKYKFVEDSEGKYSIDVESFDKLYK SLMFGFTETNIAENYKIKTRASYFS DSLPPVKIKNLLDNEIYTIEEGFNI SDKDMEKEYRGQNKAINKQAYEEIS KE
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.
• Synonyms: botB; Bontoxilysin-B
• UniProt ID: P10844

Gene Information

• Synonyms: botB; Bontoxilysin-B
• UniProt ID: P10844