Recombinant CMV UL44 Antigen, His tagged

• Cat.No.: UL44-067C
• Product Overview: The CMV antigen pp52 has been prepared as a truncated recombinant antigen fused to a his-tag in its N-terminal. It is produced from the immunological regions of the UL44 gene, which codifies the DNA polymerase processivity subunit of CMV.

Specification

• Species: CMV
• Source: E.coli
• Tag: His
• Description: pp52 (UL44) is the DNA polymerase processivity subunit of CMV. It is a phosphorylated protein, with a high presence in the virus and DNA binding capacity. pp52 corresponds to an immediate early antigen. It is one of the serological targets for detection of CMV-specific IgM.
• Tag: N-His
• Molecular Mass: Determined by SDS-PAGE, the protein band is at the molecular marker of 14.4 kDa, while relative molecular mass calculated from amino acid sequence is 11,251.1 Da.
• Purity: > 95% by observation on SDS-PAGE electrophoresis
• Applications: WB, DB, IE, DE, CLIA, LF
• Storage: Upon arrival, it should be aliquoted to avoid repeated freezing and thawing cycles and stored at -20 to -80 centigrade. In order to defrost the protein, maintain the aliquot at 25 centigrade without shaking to avoid aggregation.
• Storage Buffer: 20 mM phosphate buffer pH 8 and 0.15 M NaCl
• Concentration: 1.26 mg/mL
• Shipping: Protein is shipped with dry ice.
• Reference: 1. Maine, G.T., R. Stricker, M. Schuler, J. Spesard, S. Brojanac, B. Iriarte, K. Herwig, T. Gramins, B. Combs, J. Wise, H. Simmons, T. Gram, J. Lonze, D. Ruzicki, B. Byrne, J. D. Clifton, L. E. Chovan, D. Wachta, C. Holas, D. Wang, T. Wilson, S. Tomazic-Allen, M. A. Clements, G. L. Wright, Jr., T. Lazzarotto, A. Ripalti, andM. P. Landini. Development and Clinical Evaluation of a Recombinant-Antigen-Based Cytomegalovirus Immunoglobulin M Automated Immunoassay Using the Abbott AxSYM Analyzer. 2000, J Clin Microbiol., 38(4):1476–1481.
  2. Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.