Recombinant COVID-19 Plpro protein, His-tagged

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Recombinant COVID-19 Plpro protein, His-tagged

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Cat.No. : Plpro-198V
Product Overview : Recombinant COVID-19 Plpro Protein (Glu1564-Val1880), fused to His-tag at the N-terminus, was expressed in E.coli.
Description : The coronaviral proteases, papain-like protease (PLpro) and 3C-like protease (3CLpro), are attractive antiviral drug targets because they are essential for coronaviral replication. PLpro has the additional function of stripping ubiquitin and ISG15 from host-cell proteins to aid coronaviruses in their evasion of the host innate immune responses. Targeting PLpro with antiviral drugs may have an advantage in not only inhibiting viral replication but also inhibiting the dysregulation of signaling cascades in infected cells that may lead to cell death in surrounding, uninfected cells.
Source : E.coli
Species : COVID-19
Tag : His
Predicted N Terminal : Met
Form : Lyophilized from sterile 20mM Tris 500mM NaCl, pH 7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Molecular Mass : The recombinant SARS-CoV-2 (COVID-19) Plpro/papain-like protease (His Tag) consists of 324 amino acids and predicts a molecular mass of 36.79 kDa.
Protein length : Glu1564-Val1880
Purity : > 90% as determined by SDS-PAGE.
Stability : Samples are stable for up to twelve months from date of receipt at -20 centigrade to -80 centigrade.
Storage : Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
Shipping : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Gene Name : orf1ab
Official Symbol : orf1ab
Synonyms : GU280_gp01
Gene ID : 43740578
Protein Refseq : YP_009725299.1

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