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Recombinant COVID-19 S protein RBD(N354D), His-tagged
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Cat.No. : | Spike-213V |
Product Overview : | Recombinant COVID-19 S protein RBD (N354D, Arg319-Phe541) was fused to His-tag at the C-terminus and expressed in human 293 cells (HEK293). |
Description : | It's been reported that COVID-19 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
Source : | HEK293 |
Species : | COVID-19 |
Tag : | His |
Predicted N Terminal : | Arg319 |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose. |
Molecular Mass : | The protein has a calculated MW of 27.0 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Protein length : | Arg319-Phe541 |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents. |
Gene Name : | S |
Official Symbol : | S |
Synonyms : | spike glycoprotein |
Gene ID : | 43740568 |
Protein Refseq : | YP_009724390.1 |
UniProt ID : | P0DTC2 |
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