Active Recombinant COVID-19 Spike protein S1 subunit, His-tagged

• Cat.No.: Spike-191V
• Product Overview: Recombinant COVID-19 Spike protein S1 subunit was fused to His tag at C-terminus and expressed in human cells.

Specification

• Species: Sars-CoV-2
• Source: HEK293
• Tag: His
• Protein Length: Val16-Arg685
• Form: Lyophilized from sterile PBS, pH 7.4
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2/2019-nCoV Spike Protein (S1 Subunit, His tag), the EC50 of SARS-CoV-2/2019-nCoV Spike Protein (S1 Subunit, His tag) is 200-600 ng/mL.
  2. Loaded ACE2 Protein, Human, Recombinant (mFc Tag) on AMC Biosensor, can bind SARS-CoV-2 (2019-nCoV) Spike S1-His Recombinant Protein with an affinity constant of 47.7 nM as determined by Octet RED System.
• Molecular Mass: The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, His Tag) consists of 681 amino acids and predicts a molecular mass of 76.5 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  Broadly neutralizing aptamers to SARS-CoV-2: A diverse panel of modified DNA antiviral agents (2023)