Spike-19V
Optional Service: Optional requirements on this protein
Product Overview :
Recombinant COVID-19 Spike protein S1 subunit receptor binding domain (RBD) was fused to Mouse IgG1 Fc tag at C-terminus and expressed in human cells.
Source :
HEK293
Species :
COVID-19
Tag :
mFc
Form :
Lyophilized from sterile PBS, pH 7.4
Bio-activity :
1. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2μg/mL (100μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag), the EC50 of SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag) is 10-30 ng/mL. 2. Loaded Human ACE2 Protein (His tag) on SA Biosensor, can bind SARS-CoV-2/2019-nCoV Spike/RBD Protein (RBD, mFc Tag) with an affinity constant of 0.562 nM as determined by Octet RED System.
Molecular Mass :
The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag) consists of 457 amino acids and predicts a molecular mass of 51.5 kDa.
Protein Length :
Arg319-Phe541
Endotoxin :
< 1.0 EU per μg of the protein as determined by the LAL method
Purity :
> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
Storage :
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution :
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.