Recombinant COVID-19 Spike RBD protein, His-Avi-tagged, Biotinylated

• Cat.No.: Spike-2524V
• Product Overview: Biotinylated Recombinant COVID-19 Spike RBD protein(QHD43416.1)(Arg319-Asn532), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Sars-CoV-2
• Source: HEK293
• Tag: Avi&His
• Protein Length: Arg319-Asn532
• Tag: C-His-Avi
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated SARS-COV-2 Spike RBD, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human ACE2, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 27 kDa. Due to glycosylation, the protein migrates to 36-40 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• Conjugation: Biotin

Gene Information

• Gene Name: S spike protein [ Middle East respiratory syndrome-related coronavirus ]
• Official Symbol: S
• Synonyms: Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1
• Gene ID: 14254594
• Protein Refseq: YP_009724390.1
• UniProt ID: P0DTC2