Active Recombinant SARS-COV-2 Spike S1 protein, hFc-Avi-tagged, Low Endotoxin

• Cat.No.: Spike-2746V
• Product Overview: Active Recombinant SARS-COV-2 Spike S1 protein(QHD43416.1)(14-683 aa), fused with C-terminal hFc and Avi tag, was expressed in HEK293.

Specification

• Species: SARS-COV-2
• Source: HEK293
• Tag: Avi
• Protein Length: 14-683 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human ACE2, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for SARS-COV-2 Spike S1, hFc Tag with the EC50 of 92.3ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 102.6 kDa. Due to glycosylation, the protein migrates to 130-140 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 90% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.