Recombinant Cynomolgus ALPP protein, His-tagged

• Cat.No.: ALPP-1345C
• Product Overview: Recombinant Cynomolgus ALPP protein(XP_045223825.1)(Ile21-Thr503), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: C-His
• Protein Length: Ile21-Thr503
• Tag: C-His
• Form: Supplied as 0.22μm filtered solution in 25mM Tris, 100mM NaCl, 1mM MgCl2 (pH 7.5).
• Bio-activity: The affinity constant of 0.11 μM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 53.69 kDa. Due to glycosylation, the protein migrates to 57-67 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE..
• Storage: Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.