Recombinant Cynomolgus CD3E&CD3D, His-Fc&Flag-Fc tagged

• Cat.No.: CD3E&CD3D-220C
• Product Overview: Recombinant Cynomolgus CD3E & CD3D heterodimer protein (Cynomolgus CD3E & CD3D heterodimer) Gln 22 - Asp 117 (CD3E,Q95LI5) & Phe 22 - Ala 105 (CD3D,Q95LI8) was produced in human 293 cells (HEK293).

Specification

• Description: T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
• Source: HEK293
• Species: Cynomolgus
• Tag: His-Fc&Flag-Fc
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Molecular Mass: The heterodimer Cynomolgus CD3E & CD3D heterodimer Fc Chimera is produced by co-expression of CD3E and CD3D, has a calculated MW of 38.3 kDa (CD3E) and 37.5 kDa (CD3D). Subunit CD3E is fused with his-tagged human IgG1 Fc fragment at the C-terminus and sub
• Endotoxin: Less than 1.0 EU per μg of the Cynomolgus CD3E & CD3D heterodimer Fc Chimera by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
• Reconstitution: See Certificate of Analysis for reconstitution instructions and specific concentrations.

Gene Information

• Gene Name: CD3E CD3e molecule, epsilon (CD3-TCR complex) [ Macaca fascicularis (crab-eating macaque) ]
• Official Symbol: CD3E
• Synonyms: FLJ18683, T3E, TCRE, CD3E, CD3-epsilon, CD3 epsilon, T-cell surface glycoprotein CD3 epsilon chain, CD3 epsilon FN18+, CD3 epsilon FN18-
• Gene ID: 102133065
• mRNA Refseq: NM_001283615.1
• Protein Refseq: NP_001270544.1
• UniProt ID: Mfa.6117
• Pathway: Chagas disease (American trypanosomiasis), organism-specific biosystem; HTLV-I infection, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem; Measles, organism-specific biosystem; Primary immunodeficiency, organism-specific biosystem; T cell receptor signaling pathway, organism-specific biosystem