Recombinant Cynomolgus CD3E & CD3D protein, Fc-His-tagged & Fc-Flag-tagged, Biotinylated
Cat.No. : | CD3E & CD3D-515CB |
Product Overview : | Cynomolgus CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # Q95LI5-1(CD3E) & Q95LI8-1(CD3D)). |
- Specification
- Gene Information
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Description : | T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. |
Source : | HEK293 |
Species : | Cynomolgus |
Tag : | Fc-His & Fc-Flag |
Predicted N Terminal : | Gln 22 (CD3E) & Phe 22 (CD3D) |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Molecular Mass : | Cynomolgus CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 42.5 kDa (CD3E) and 40.9 kDa (CD3D). Subunit CD3E is fused with a human IgG1 Fc tag and a polyhistidine tag at the C-terminus and subunit CD3D is fused with a human IgG1 Fc tag and a flag tag at the C-terminus. The predicted N-terminus is Gln 22 (CD3E) & Phe 22 (CD3D). The reducing (R) protein migrates as 46-50 kDa and 55-65 kDa due to glycosylation. |
Protein length : | Gln 22 - Asp 117 (CD3E) & Phe 22 - Ala 105 (CD3D) |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >90% as determined by SDS-PAGE. >90% as determined by SEC-MALS. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Gene Name : | Cd3e CD3 antigen, epsilon polypeptide [ Mus musculus (house mouse) ] |
Official Symbol : | CD3E; CD3D |
Synonyms : | CD3E & CD3D; CD3 delta & CD3 epsilon; CD3 delta / epsilon |
Gene ID : | 12501 |
mRNA Refseq : | NM_007648.5 |
Protein Refseq : | NP_031674.1 |
UniProt ID : | P22646 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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