Recombinant Cynomolgus CD3E&CD3D protein, FLAG-Fc-tagged
Cat.No. : | CD3E&CD3D-209C |
Product Overview : | Recombinant Cynomolgus CD3D protein (Q95LI8-1)(Met1-Ala105) with the C-terminal his-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; Recombinant Cynomolgus CD3E protein(XP_015290838.1) (Met1-Asp117) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the CD3D&CD3E heterodimer was purified. |
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Source : | HEK293 |
Species : | Cynomolgus |
Tag : | Fc&Flag |
Form : | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Molecular Mass : | The recombinant heterodimer of cynomolgus CD3D&CD3E comprises 679 (334+345) amino acids and has a calculated molecular mass of 76.63 (37.75+ 38.88) KDa. The apparent molecular mass of rhesus CD3D&CD3E heterodimer is approximately (50.99 & 40.83) KDa respectively in SDS-PAGE. |
Protein length : | Met1-Ala105&Met1-Asp117 |
Endotoxin : | < 1.0 EU per μg protein as determined by the LAL method. |
Purity : | > 95 % as determined by SDS-PAGE. |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
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◆ Lysates | ||
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CD3E-1602CCL | Recombinant Cynomolgus CD3E cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Customer Reviews (3)
Write a reviewManufacturers with the capacity for bulk production can offer CD3E protein in larger quantities, which is advantageous for researchers working on large-scale experiments or collaborations.
bulk production often leads to economies of scale, making the CD3E protein more cost-effective for research budgets.
Manufacturers can actively seek collaborations and partnerships with researchers to develop new applications or optimize methodologies using CD3E protein.
Q&As (5)
Ask a questionYes, CD3E levels and T-cell function can be monitored to evaluate the response to cancer immunotherapies like checkpoint inhibitors.
There are currently no approved therapies that directly target CD3E, but it is a target for research in developing immunotherapies and cancer treatments.
There may be ongoing clinical trials using CD3E-targeted therapies in the context of cancer and autoimmune diseases. It's important to check clinical trial databases for specific studies.
Yes, monitoring CD3E levels in HIV-infected individuals can provide valuable information about the progression of the disease and the effectiveness of antiretroviral therapy.
CD3E can be used in research to study T-cell responses in allergic conditions, helping to better understand and diagnose allergies.
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