Recombinant Cynomolgus CD3E & CD3D protein, hFc-tagged

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Recombinant Cynomolgus CD3E & CD3D protein, hFc-tagged

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Cat.No. : CD3E & CD3D-203C
Product Overview : Cynomolgus CD3E&CD3D Protein is expressed from mammalian with a hFc Tag at the C-terminal. It contains Asp20-Arg687 (Q95LI5. 2 ) & Phe22-Ala105 (Q95LI8).
Description : T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3D , are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Source : HEK293
Species : Cynomolgus
Tag : hFc
Form : Supplied as 0. 22um filtered solution in PBS (pH 7. 4).
Molecular Mass : The protein has a predicted MW of 36. 9 kDa (CD3E)and 35. 4 kDa (CD3D). Due to glycosylation, the protein migrates to 58-62KDa and 44-46KDa based on Bis-Tris PAGE result.
Protein length : It contains Asp20-Arg687 (Q95LI5. 2 ) & Phe22-Ala105 (Q95LI8).
Endotoxin : Less than 0. 001EU per ug by the LAL method.
Purity : > 95% as determined by SDS-PAGE; > 95% as determined by HPLC
Storage : The product should be stored at -70°C. Please do not repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Tris-Bis PAGE :

HPLC data :

ELISA data :

Gene Name : CD3E CD3e molecule [ Macaca fascicularis (crab-eating macaque) ]
Official Symbol : CD3E; CD3D
Synonyms : CD3;CD3D;CD3-DELTA;T3D;CD3G
Gene ID : 102133065
mRNA Refseq : XM_015435352. 1
Protein Refseq : XP_015290838. 1
UniProt ID : Q95LI5

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