Recombinant Cynomolgus CD3E&CD3D protein, hFc-tagged, Biotinylated

• Cat.No.: CD3E & CD3D-203CB
• Product Overview: Biotinylated Recombinant Cynomolgus CD3E&CD3D protein(Q95LI5.2(CD3E)&Q95LI8(CD3D))(Gln22-Asp117(CD3E) & Phe22-Ala105(CD3D)), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Fc
• Protein Length: Gln22-Asp117(CD3E) & Phe22-Ala105(CD3D)
• Tag: C-hFc
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Anti-CD3 Antibody, hFc Tag at 2μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E&CD3D, hFc Tag with the EC50 of 0.10μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 36.9 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation, the protein migrates to 58-62 kDa and 44-46 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• Conjugation: Biotin

Gene Information

• Official Symbol: CD3E
• Official Symbol 2: CD3D