Species : |
Cynomolgus |
Source : |
HEK293 |
Tag : |
Fc |
Protein Length : |
Gln22-Asp117(CD3E) & Phe22-Ala105(CD3D) |
Tag : |
C-hFc |
Conjugation/Label : |
Biotin |
Form : |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
Bio-activity : |
Immobilized Anti-CD3 Antibody, hFc Tag at 2μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E&CD3D, hFc Tag with the EC50 of 0.10μg/ml determined by ELISA. |
Molecular Mass : |
The protein has a predicted MW of 36.9 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation, the protein migrates to 58-62 kDa and 44-46 kDa based on Tris-Bis PAGE result. |
Endotoxin : |
Less than 1EU per μg by the LAL method. |
Purity : |
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Storage : |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Reconstitution : |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |