Recombinant Cynomolgus CD3E&CD3G protein, hFc-tagged, Biotinylated

• Cat.No.: CD3E&CD3G-6754C
• Product Overview: Biotinylated Recombinant Cynomolgus CD3E&CD3G protein(Q95LI5.2(CD3E)&Q95LI7(CD3G))(Gln22-Asp117(CD3E) & Gln23-Thr113(CD3G)), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Fc
• Protein Length: Gln22-Asp117(CD3E) & Gln23-Thr113(CD3G)
• Tag: C-hFc
• Conjugation/Label: Biotin
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Anti-CD3 Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E&CD3G, hFc Tag with the EC50 of 50.5ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 36.9 kDa (CD3E) and 36.5 kDa (CD3G). Due to glycosylation, the protein migrates to 44-46 kDa&46-48 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C; Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Conjugation: Biotin

Gene Information

• Official Symbol: CD3E
• Official Symbol 2: CD3G