Recombinant Cynomolgus CD8A & CD8B protein(Met42-Asp223 & Met1-Pro170), Flag&His-tagged

• Cat.No.: CD8A & CD8B-1265C
• Product Overview: Recombinant Cynomolgus CD8A (XP_001092778.2) (Met42-Asp223) was fused with a flag tag at the C-terminus, constructed the plasmid 1 ; Recombinant Cynomolgus CD8B (XP_005575417.1) (Met1-Pro170) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified and expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Flag&His
• Protein Length: Met42-Asp223 & Met1-Pro170
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant Cynomolgus CD8A & CD8B heterodimer comprises 395 (202+193) amino acids and has a calculated molecular mass of 44.27 (22.51+21.76) kDa. It migRates as an approximately 33.52 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Official Symbol: CD8A; CD8B