Recombinant Cynomolgus DDR2, His tagged

• Cat.No.: DDR2-120C
• Product Overview: Recombinant Cynomolgus DDR2 (F7HJQ2) (Met1-Arg399), fused with a polyhistidine tag at the C-terminus, was produced in Human Cells.

Specification

• Species: Cynomolgus
• Source: Human Cells
• Tag: His
• Protein Length: 1-399 a.a.
• Predicted N Terminal: Lys 22
• Form: Lyophilized from sterile PBS, pH 7.4.Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Molecular Mass: The recombinant cynomolgus DDR2 comprises 389 amino acids and has a calculated molecular mass of 44.1 KDa. The apparent molecular mass of it is approximately 55 KDa respectively in SDS-PAGE.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
• Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: DDR2?discoidin domain receptor tyrosine kinase 2 [?Macaca fascicularis?(crab-eating macaque) ]
• Official Symbol: DDR2
• Gene ID: 102128344
• mRNA Refseq: XM_005541125
• Protein Refseq: XP_005541182
• Chromosome Location: 1

Citation

Collagen Advanced Glycation Inhibits Its Discoidin Domain Receptor 2 (DDR2)-Mediated Induction of Lysyl Oxidase in Osteoblasts

Journal: Bone    PubMed ID: 24120383    Data: 2015/1/1

Authors: Roozbeh Khosravi, Katharine L. Sodek, Philip C. Trackman

Article Snippet:After washing (PBS containing 0.05% Tween 20), wells were blocked with 150 μL of 1 mg/ml BSA in PBS for 1 hour at room temperature.After washing (PBS containing 0.05% Tween 20), wells were blocked with 150 μL of 1 mg/ml BSA in PBS for 1 hour at room temperature.. Recombinant DDR2-(His) 6 (30 pmole) (Creative BioMart cat#773M) was serially diluted in the incubation buffer (PBS with 0.5 mg/ml BSA) in a final volume of 50 μL and added to each well.. The plate was incubated at room temperature for 3 hours on a gently shaking horizontal rotator.The plate was incubated at room temperature for 3 hours on a gently shaking horizontal rotator.