Recombinant Cynomolgus HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer protein, His-Avi-tagged, Biotinylated

• Cat.No.: HLA-G&B2M-1733C
• Product Overview: Recombinant Cynomolgus HLA-G Tetramer Protein(Gly25-Thr305(HLA-G), Ile21-Met119(B2M) and RIIPRHLQL peptide) is expressed from HEK293 with His tag and Avi tag at the C-terminal, tetramer is assembled by Biotinylated monomer and streptavidin.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Avi&His
• Protein Length: Gly25-Thr305(HLA-G), Ile21-Met119(B2M)and RIIPRHLQL peptide
• Tag: C-His-Avi
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Cynomolgus LILRB2, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag with an affinity constant of 42.50 nM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• Conjugation: Biotin