Recombinant Cynomolgus IL3RA protein, His-tagged

• Cat.No.: IL3RA-179C
• Product Overview: Recombinant Cynomolgus IL3RA protein(G8F3K3-1)(Arg18-Arg302), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• Protein Length: Arg18-Arg302
• Tag: C-His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Human IL-3, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus IL-3 R alpha, His Tag with an affinity constant of 0.46 μM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 33.9 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.