Recombinant Cynomolgus LGALS3 protein, His-tagged

• Cat.No.: LGALS3-1459C
• Product Overview: Recombinant Cynomolgus LGALS3 protein(XP_005561371.1)(Ala2-Ile248), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: N-His
• Protein Length: Ala2-Ile248
• Tag: N-His
• Form: Supplied as 0.22μm filtered solution in 50mM Hepes, 150mM NaCl, 200mM L-Arginine, 2mM Tcep (pH 6.5).
• Bio-activity: Immobilized Cynomolgus Galectin 3, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Galectin 3 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 27.05 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE.
• Storage: Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.