Species : |
Cynomolgus |
Source : |
HEK293 |
Tag : |
His |
Protein Length : |
Pro22-Asn254 |
Tag : |
C-His |
Form : |
Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM NaCl, 100mM L-Arginine (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization. |
Bio-activity : |
Immobilized Cynomolgus NKp46, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-NKp46 Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA (QC Test). |
Molecular Mass : |
The protein has a predicted MW of 27.4 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result. |
Endotoxin : |
Less than 1EU per μg by the LAL method. |
Purity : |
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Storage : |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Reconstitution : |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |