Recombinant Cynomolgus/Rhesus macaque CD80 protein, Fc-tagged

• Cat.No.: CD80-137C
• Product Overview: Recombinant Cynomolgus/Rhesus macaque CD80 (Val35-Asn242) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Species: Cynomolgus/Rhesus macaque
• Source: HEK293
• Tag: Fc
• Protein Length: Val35-Asn242
• Description: B7-1 and B7-2, together with their receptors CD28 and CTLA­4, constitute one of the dominant co-stimulatory pathways that regulate T­ and B­cell responses. Although both CTLA­4 and CD28 can bind to the same ligands, CTLA­4 binds to B7­1 and B7­2 with a 20 ­ 100 fold higher affinity than CD28 and is involved in the down­regulation of the immune response.
  B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
• Predicted N Terminal: Val35
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 50.5 kDa. The protein migrates as 60-90 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by reduced SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CD80
• Official Symbol: CD80
• Synonyms: CD80; CD80 antigen; T-lymphocyte activation antigen CD80; B7 protein; activation B7-1 antigen; B71; Ly53; TSA1; Cd28l; Ly-53; MIC17
• Gene ID: 102140078
• mRNA Refseq: XM_015446388.1
• Protein Refseq: XP_015301874.1
• UniProt ID: F7HRG1