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Recombinant Cynomolgus THPO Protein, His-tagged

Cat.No.: THPO-1163C
Product Overview: Recombinant Cynomolgus (Macaca fascicularis) THPO (Met 1-Gly 353) precursor was expressed, with a C-terminal polyhistidine tag.
Source: HEK293
Species: Cynomolgus
Tag: His
Predicted N Terminal: Ser 22
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity: Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 1-5 ng/ml.
Molecular Mass: The recombinant cynomolgus THPO consists of 343 amino acids and has a calculated molecular mass of 36.8 kDa. The apparent molecular mass of the recombinant protein is approximately 70-80 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >86 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: THPO thrombopoietin [ Macaca fascicularis ]
Official Symbol: THPO
Synonyms: thrombopoietin
Gene ID: 102144240
mRNA Refseq:
Protein Refseq:
MIM:
UniProt ID:
Chromosome Location: chromosome: 2
Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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