Recombinant Cynomolgus TNFSF13 protein, His-Avi,Flag-tagged

• Cat.No.: TNFSF13-754C
• Product Overview: Recombinant Cynomolgus TNFSF13 protein(A0A2K5TJA1)(Lys112-Leu250), fused with N-terminal His and Avi,N-terminal Flag tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Avi&Flag&His
• Protein Length: Lys112-Leu250
• Tag: N-His-Avi,N-Flag
• Form: Supplied as 0.22μm filtered solution in 50mM Tris, 150mM NaCl, 200mM L-arginine (pH 8.0).
• Bio-activity: Immobilized Cynomolgus APRIL (Trimer) , His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque BCMA, hFc Tag with the EC50 of 1.9 ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 52.1 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.