Active Recombinant Cynomolgus TNFSF13 protein, His-Avi-Flag-tagged, Biotinylated, Low Endotoxin

• Cat.No.: TNFSF13-754CB
• Product Overview: Biotinylated Recombinant Cynomolgus TNFSF13 protein(A0A2K5TJA1)(112-250 aa), fused with N-terminal His, Avi and Flag tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Avi&Flag&His
• Protein Length: 112-250 aa
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, 200 mM L-arginine (pH 8.0). Normally 8% mannitol is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Cynomolgus APRIL (Trimer), His-Avi Tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Cynomolgus BCMA, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 52.1 kDa. Due to glycosylation, the protein migrates to 56-65 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 90% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.