Recombinant Cynomolgus TSLP protein, His-tagged TSLP-255C

Recombinant Cynomolgus TSLP protein, His-tagged

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Recombinant Cynomolgus TSLP protein, His-tagged

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Cat.No. : TSLP-255C
Product Overview : Recombinant Cynomolgus TSLP protein (Tyr29-Gln159) was fused to His-tag at the C-terminus and expressed in human 293 cells (HEK293).
Description : Thymic stromal lymphopoietin (TSLP) is an epithelial cell-derived cytokine involved in the pathology of inflammatory skin diseases, and is widely expressed by epithelial cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence. Human TSLP has been shown to developing nondeletional central tolerance, amplifying epithelium-induced class switching, inducing atopic diseases and maintaining intestinal noninflammatory environment. Among diverse cells responding to Human TSLP, CD11c+ dendritic cells are the most obviously characterized target cells.
Source : HEK293
Species : Cynomolgus
Tag : His
Predicted N Terminal : Tyr29
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : This protein contains a furin convertase cleavage site, 127-RRKR-130, and will be processed into N and C-terminal fragment with calculated MW of 11.7 kDa and 5.4 kDa respectively. The protein migrates as 21 kDa, 15 kDa and 8 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Tyr29-Gln159
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
SDS-PAGE of TSLP-255C :

Cynomolgus TSLP, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA of TSLP-255C :

Immobilized Cynomolgus TSLP, His Tag at 5 μg/mL (100 μL/well) can bind Human TSLP R, Fc Tag with a linear range of 2-31 ng/mL (QC tested).

Immobilized Cynomolgus TSLP, His Tag at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human TSLP Antibody, Human IgG2 with a linear range of 1-16 ng/mL (Routinely tested).

Gene Name : TSLP
Official Symbol : TSLP
Synonyms : TSLP
Gene ID : 102119254
mRNA Refseq : XM_005557498.2
Protein Refseq : XP_005557555.1
UniProt ID : A0A2K5TXV0

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