- Cynomolgus(1)
- Human(14)
- Mouse(6)
- Rat(1)
Recombinant Cynomolgus TSLP protein, His-tagged
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| Cat.No. : | TSLP-255C |
| Product Overview : | Recombinant Cynomolgus TSLP protein (Tyr29-Gln159) was fused to His-tag at the C-terminus and expressed in human 293 cells (HEK293). |
| Description : | Thymic stromal lymphopoietin (TSLP) is an epithelial cell-derived cytokine involved in the pathology of inflammatory skin diseases, and is widely expressed by epithelial cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence. Human TSLP has been shown to developing nondeletional central tolerance, amplifying epithelium-induced class switching, inducing atopic diseases and maintaining intestinal noninflammatory environment. Among diverse cells responding to Human TSLP, CD11c+ dendritic cells are the most obviously characterized target cells. |
| Source : | HEK293 |
| Species : | Cynomolgus |
| Tag : | His |
| Predicted N Terminal : | Tyr29 |
| Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose. |
| Molecular Mass : | This protein contains a furin convertase cleavage site, 127-RRKR-130, and will be processed into N and C-terminal fragment with calculated MW of 11.7 kDa and 5.4 kDa respectively. The protein migrates as 21 kDa, 15 kDa and 8 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Protein length : | Tyr29-Gln159 |
| Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
| Purity : | >90% as determined by SDS-PAGE. |
| Storage : | For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| SDS-PAGE of TSLP-255C : |
Cynomolgus TSLP, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%. |
| Bioactivity-ELISA of TSLP-255C : |
Immobilized Cynomolgus TSLP, His Tag at 5 μg/mL (100 μL/well) can bind Human TSLP R, Fc Tag with a linear range of 2-31 ng/mL (QC tested). 
Immobilized Cynomolgus TSLP, His Tag at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human TSLP Antibody, Human IgG2 with a linear range of 1-16 ng/mL (Routinely tested). |
| Gene Name : | TSLP |
| Official Symbol : | TSLP |
| Synonyms : | TSLP |
| Gene ID : | 102119254 |
| mRNA Refseq : | XM_005557498.2 |
| Protein Refseq : | XP_005557555.1 |
| UniProt ID : | A0A2K5TXV0 |
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◆ Lysates
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